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Centromeric plasmids and artificial chromosomes: new kids on the Plasmodium transfection block

Jean-Christophe Barale1, Robert Ménard

  • 1Unité d'Immunologie Moléculaire des Parasites, URA CNRS 2581, Département de Parasitologie et de Mycologie, Institut Pasteur, Paris, France.

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Plasmids

Plasmids are extrachromosomal DNA molecules found in bacteria, archaea, and some eukaryotic microbes like yeast. These small, circular DNA structures typically contain fewer than 30 genes, although some may exist linearly. Plasmids vary in their number within a cell, known as copy number. Single-copy plasmids are present in one copy per cell and multi-copy plasmids are present in multiple copies, reaching over 100 copies per cell.Plasmids usually replicate independently of the chromosomal DNA...