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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

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Related Experiment Video

Updated: Jun 15, 2026

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
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Diversity-oriented fluorescence library approaches for probe discovery and development.

Marc Vendrell1, Jun-Seok Lee, Young-Tae Chang

  • 1Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), 138667 Singapore, Singapore.

Current Opinion in Chemical Biology
|March 16, 2010
PubMed
Summary
This summary is machine-generated.

Diversity-oriented fluorescence library approaches accelerate new sensor development using combinatorial chemistry and high-throughput screening. These methods are crucial for creating advanced fluorescent probes for diverse biological applications.

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Area of Science:

  • Chemical Biology
  • Molecular Imaging
  • Biotechnology

Background:

  • Designing specific fluorescent probes is challenging.
  • Traditional probe design methods have limitations.
  • Diversity-oriented approaches offer a powerful alternative.

Purpose of the Study:

  • To review the impact of diversity-oriented fluorescence library approaches on sensor development.
  • To highlight the role of combinatorial chemistry and high-throughput screening.
  • To discuss the future potential of these methods in probe discovery.

Main Methods:

  • Combinatorial chemistry for scaffold derivatization and property tuning.
  • High-throughput screening platforms (in vitro assays, cell-based imaging).
  • Optimization of image acquisition and analysis for complex biological systems.

Main Results:

  • Successful generation of diverse fluorescent sensor libraries.
  • Demonstrated applications in sensing biomolecules, proteins, and phenotypes.
  • Expansion of screening capabilities to demanding biological systems.

Conclusions:

  • Diversity-oriented fluorescence library approaches are vital for advancing sensor technology.
  • These methods overcome limitations in rational probe design.
  • Continued innovation in these areas will drive future discoveries in fluorescent probe development.