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Updated: Jun 15, 2026

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies
09:32

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies

Published on: September 23, 2014

Tissue microarray constructs to predict a response to chemoradiation in rectal cancer.

Sergio Huerta1, John Hrom, Xiaohuan Gao

  • 1Department of Surgery, University of Texas Southwestern Medical Center, United States. Sergio.Huerta@UTSouthwestern.edu

Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver
|March 16, 2010
PubMed
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This study identified biomarkers in rectal tumors that predict response to chemoradiation. BAX and MIB are key indicators, with MIB independently predicting treatment success.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Rectal cancer treatment often involves neoadjuvant chemoradiation.
  • Predicting treatment response is crucial for personalized therapy.
  • Identifying predictive biomarkers can optimize patient management.

Purpose of the Study:

  • To identify an immunohistochemical panel using tissue microarray (TMA) that predicts response to ionizing radiation (IR) in rectal cancer.
  • To evaluate specific protein markers associated with treatment outcomes.

Main Methods:

  • Tissue microarray (TMA) constructs from 38 rectal tumors and adjacent mucosa were analyzed.
  • Immunohistochemistry (IHC) was performed for markers including MIB, Cyclin E, p53, Bcl-2, and BAX.
  • Univariate and logistic regression analyses were used to identify predictive factors.

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Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing
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Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing

Published on: September 25, 2011

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Last Updated: Jun 15, 2026

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies
09:32

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies

Published on: September 23, 2014

Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing
15:17

Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing

Published on: September 25, 2011

Main Results:

  • Pathological complete response (pCR) rate was 23.9%.
  • Immunoreactivity for MIB, p53, Bcl-2, and BAX was significantly associated with pathological response.
  • BAX showed the highest sensitivity (92%), while MIB independently predicted response (p=0.001).

Conclusions:

  • A combined panel of apoptosis mediators and clinical factors can predict chemoradiation response in rectal cancer.
  • MIB is an independent predictor of response to chemoradiation.
  • This approach is feasible for rectal tumor biopsies to guide treatment decisions.