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Updated: Jun 15, 2026

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
Published on: December 1, 2018
Fluorescence fluctuations of quantum-dot sensors capture intracellular protein interaction dynamics
Eli Zamir1, Piet H M Lommerse, Ali Kinkhabwala
1Department of Systemic Cell Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany.
Abstract:
We extend the in vitro principle of co-immunoprecipitation to quantify dynamic protein interactions in living cells. Using a multiresolution implementation of fluorescence correlation spectroscopy to achieve maximal temporal resolution, we monitored the interactions of endogenous bait proteins, recruited by quantum dots, with fluorescently tagged prey. With this approach, we analyzed the rapid physiological regulation of protein kinase A.
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