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Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Related Experiment Video

Updated: Jun 15, 2026

Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases In Vivo
10:51

Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases In Vivo

Published on: October 31, 2014

Study on gene sensor based on primer extension.

Y Chen1, J Song, D Li

  • 1Key Laboratory of Cell Biology, Ministry of Public Health of China, China Medical University, 110001, Shenyang, China.

Science in China. Series C, Life Sciences
|March 16, 2010
PubMed
Summary
This summary is machine-generated.

A novel primer extension gene sensor uses piezoelectric crystal frequency shifts to detect gene hybridization and extension. This sensitive method enables rapid DNA detection and analysis for various genetic applications.

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Last Updated: Jun 15, 2026

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Biosensing

Background:

  • Piezoelectric crystal resonant frequency is sensitive to surface mass changes.
  • Primer extension is a key step in many molecular biology techniques.
  • Existing gene detection methods can lack speed or sensitivity.

Purpose of the Study:

  • To develop a novel gene sensor technique utilizing primer extension and piezoelectric crystals.
  • To leverage frequency signals for sensitive monitoring of gene hybridization and primer extension.
  • To explore applications in nucleic acid analysis and DNA detection.

Main Methods:

  • Developed a gene sensor based on piezoelectric crystal resonant frequency.
  • Utilized primer extension coupled with hybridization to a DNA template.
  • Monitored hybridization and extension events via changes in crystal frequency signals.

Main Results:

  • Demonstrated fast and sensitive detection of gene hybridization and primer extension.
  • Showcased the technique's potential for homologous nucleic acid analysis.
  • Confirmed utility in trace DNA detection and DNA integration determination.

Conclusions:

  • The primer extension gene sensor offers a rapid and sensitive platform for genetic analysis.
  • Potential applications include gene isolation, mutation analysis, and genomic location prediction.
  • This technique advances biosensing for molecular diagnostics and research.