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Updated: Jun 15, 2026
Establishing a Device for Sleep Deprivation in Mice
Published on: September 22, 2023
[Effects of complete and incomplete sleep deprivation on immune function of mice]
Tian-yang Yang1, Yan-hua Huang, Hao-min Cai
1Shanghai Institute of Immunology, Shanghai Jiaotong University Basic Medical School, Shanghai, China. yannikyangtianyang@hotmail.com
Aim:
To study the interaction between sleep deprivation and immune functions in mice.
Methods:
To set up the uncompleted sleep deprivation of mice by means of lighting mimicked with whole day and dark box done with whole night for 2 weeks and to deprive the mice of sleep in the rotating cage for 24 h and 72 h. All mice are challenge with BSA before sleep deprivation. The spleen weight of mice was measured by analytic balance. The count of T subpopulation was detected with FACS. The levels of cytokines IL-2, IL-10 and the concentration of the specific antibody to BSA was detected by ELISA method.
Results:
Compared with the normal control, the spleen weight of mice in all the other experimental groups was decreased (P<0.05) except those of lighting group. Decrease of CD8+ T lymphocyte was observed (P<0.01) while the ratio CD4/CD8 increased greatly. Augmentation of IL-10 was demonstrated only in the all-light group, both IL-2 and IL-10 decreased (P<0.01) in the other groups. Significant decrease of specific antibody for BSA was found in the mice of sleep deprived group (P<0.01).
Conclusion:
Complete and incomplete sleep deprivation inhibit differently the immune function of mice by triggering decrease of CD8+ T cells, inhibiting secretion of specific antibody and causing disturbance on the pattern of cytokines.
