Related Experiment Video
Updated: Jun 15, 2026
Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
Published on: January 17, 2015
[Cloning of human scFv against soman transition state analogues (P6) from a large phage antibody library]
Yu-xiao Wang1, Pei-yuan Jia, Yu-xia Wang
1Central Laboratory Navy General Hospital, Beijing, China.
Aim:
To clone human anti-soman transition state analogues antibodies from a large single-chain phage antibody library.
Methods:
An organophosphorus hapten P6 [O1-methyl-O2-(1, 2, 2-trimethylpropyl)-2-hydroxy-5-nitrophenyl methylphosphonic acid] was synthesized. Its chemical conjugate with bovine serum albumin (BSA) was used as antigen (P6-BSA) to screen antibodies against soman. Panning of a large single-chain phage antibody library was conducted to select specific antibodies against soman. The antigen binding characteristics were analyzed by ELISA.
Results:
After 4 rounds of panning, 14 clones had specific binding ability to P6. DNA fingerprinting showed that diverse specific human scFvs against P6 was obtained from the library by biopanning.
Conclusion:
Human anti-soman transition state analogues scFvs have been cloned from large phage antibody library.
