Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Formation of Muscle Fibers from Myoblasts01:13

Formation of Muscle Fibers from Myoblasts

De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription factors...
Forced Transdifferentiation01:28

Forced Transdifferentiation

Transdifferentiation, also known as lineage reprogramming, was first discovered by Selman and Kafatos in 1974 in silkmoths. They observed that the moths’ cuticle-producing cells transformed into salt-producing cells. Many such cases of natural transdifferentiation occur in organisms. In humans, pancreatic alpha cells can become beta cells. In newts, the loss of the eye’s lens causes the pigmented epithelial cells to transdifferentiate into the lens cells.
Artificial transdifferentiation occurs...
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.
Zygotic Development And Stem Cell Formation01:10

Zygotic Development And Stem Cell Formation

The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Paediatric Therapeutic Development Workshop on rhabdomyosarcoma.

British journal of cancer·2026
Same author

Dynamic Reorganization of Developmental to Adult Genome Topology Controls the Initiation and Stabilization of the Human Muscle Stem Cell State.

bioRxiv : the preprint server for biology·2026
Same author

Author Correction: Long non-coding RNA Linc-RAM enhances myogenic differentiation by interacting with MyoD.

Nature communications·2025
Same author

Tenascin-C from the tissue microenvironment promotes muscle stem cell maintenance and function through Annexin A2.

Communications biology·2025
Same author

Modulation of the JAK2-STAT3 pathway promotes expansion and maturation of human iPSC-derived myogenic progenitor cells.

Stem cell reports·2025
Same author

Advancements in Nonviral Gene Editing Strategies for Rare Diseases.

Human gene therapy·2025
Same journal

AXIN1 and AXIN2 regulate the WNT-signaling landscape to promote distinct mesoderm programs.

Developmental cell·2026
Same journal

ARID1A terminates gastric regeneration to prevent cancer.

Developmental cell·2026
Same journal

Myc sustains sex-biased organ zonation in the Drosophila intestine.

Developmental cell·2026
Same journal

Two parallel neuronal circuits involving electrical synapse and DAF-7/TGF-β signaling regulate muscle autophagy in C. elegans.

Developmental cell·2026
Same journal

Menstruation: Once unspoken but now uncovered, one cell type at a time.

Developmental cell·2026
Same journal

The ALS- and FTD-associated proteins annexin A11 and CHMP2B act sequentially in plasma membrane repair.

Developmental cell·2026
See all related articles

Related Experiment Video

Updated: Jun 15, 2026

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
14:47

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Published on: May 17, 2016

Switch NFix developmental myogenesis.

Daniela Palacios1, Pier Lorenzo Puri

  • 1Dulbecco Telethon Institute , IRCCS Fondazione Santa Lucia and European Brain Research Institute, 00161 Roma, Italy.

Developmental Cell
|March 17, 2010
PubMed
Summary
This summary is machine-generated.

Researchers discovered NFix, a key transcription factor, which controls gene expression changes in skeletal muscle development. This finding explains how muscles adapt to different stages from embryonic to fetal development.

More Related Videos

In Vitro Differentiation of Mature Myofibers for Live Imaging
08:12

In Vitro Differentiation of Mature Myofibers for Live Imaging

Published on: January 7, 2017

Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells
14:36

Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells

Published on: September 21, 2010

Related Experiment Videos

Last Updated: Jun 15, 2026

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
14:47

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Published on: May 17, 2016

In Vitro Differentiation of Mature Myofibers for Live Imaging
08:12

In Vitro Differentiation of Mature Myofibers for Live Imaging

Published on: January 7, 2017

Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells
14:36

Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells

Published on: September 21, 2010

Area of Science:

  • Muscle development
  • Gene regulation
  • Developmental biology

Background:

  • Skeletal muscles undergo significant gene expression changes during development.
  • These adaptations are crucial for meeting stage-specific functional and metabolic demands.
  • The underlying molecular mechanisms governing these transitions remain largely unknown.

Discussion:

  • Messina and colleagues identified NFix as a critical transcription factor.
  • NFix plays a central role in coordinating gene expression.
  • This coordination is essential for the transition from embryonic to fetal myoblasts.

Key Insights:

  • Identification of NFix as a master regulator of skeletal muscle gene expression.
  • Elucidation of a mechanism controlling developmental gene switching.
  • Provides a molecular basis for muscle adaptation during embryogenesis.

Outlook:

  • Further investigation into NFix's downstream targets and regulatory networks.
  • Exploring potential therapeutic applications targeting NFix in muscle disorders.
  • Understanding the broader implications for developmental gene regulation.