Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transmission Electron Microscopy01:15

Transmission Electron Microscopy

In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400 keV in...
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dual Functionality of a Plasmonic Gold Photocatalyst in the Reversible Dehydrogenation / Hydrogenation of N-Heterocycles.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Simultaneous Laser Reduction of Sn/Sb Salts and Graphene Formation as Innovative Anode Material for Li- and Na-Ion Batteries.

Global challenges (Hoboken, NJ)·2026
Same author

Low levels of the second messenger c-di-GMP enhance tolerance and resistance to meropenem in <i>Pseudomonas aeruginosa</i>.

Frontiers in cellular and infection microbiology·2026
Same author

Morphology, local stoichiometry, and photoexcited states in Cu@Cu2O nanostructured systems grown by physical synthesis.

The Journal of chemical physics·2026
Same author

Mitigation of acrylamide in cookies through partial replacement of conventional sugars with erythritol and maltitol.

Food chemistry·2026
Same author

DC-magnetometry Analytical Tool Driven by Spin Ordering Phenomena for Sensing Chemical Interactions at the Surface of Nanomaterials.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Related Experiment Video

Updated: Jun 15, 2026

Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy
13:13

Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy

Published on: December 3, 2012

Electron microscopy studies of electron-beam sensitive PbTe-based nanostructures.

Andrea Falqui1, Giovanni Bertoni, Alessandro Genovese

  • 1Istituto Italiano di Tecnologia, Via Morego 30, Genoa, Italy. andrea.falqui@iit.it

Microscopy Research and Technique
|March 17, 2010
PubMed
Summary

Researchers synthesized lead telluride (PbTe) nanocrystals and reacted them with gold(III) chloride (AuCl(3)) to create diverse nanostructures. These structures, including gold-coated and unique mushroom-shaped forms, showed sensitivity to electron beam irradiation.

More Related Videos

Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy
09:09

Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy

Published on: August 10, 2019

Related Experiment Videos

Last Updated: Jun 15, 2026

Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy
13:13

Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy

Published on: December 3, 2012

Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy
09:09

Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy

Published on: August 10, 2019

Area of Science:

  • Materials Science
  • Nanotechnology
  • Chemistry

Background:

  • Lead telluride (PbTe) nanocrystals are of interest for various applications.
  • Controlling the synthesis of novel nanostructures is crucial for materials development.

Purpose of the Study:

  • To synthesize and characterize novel nanostructures derived from PbTe nanocrystals.
  • To investigate the influence of reaction conditions on the resulting nanostructures.
  • To study the stability and behavior of these nanostructures under electron beam irradiation.

Main Methods:

  • Synthesis of PbTe nanocrystals.
  • Reaction of PbTe nanocrystals with a toluene solution of AuCl(3).
  • Characterization using C(s)-corrected High Resolution Transmission Electron Microscopy (HRTEM) and Scanning Transmission Electron Microscopy (STEM).
  • Compositional analysis using Energy Dispersive X-Ray Spectrometry (EDX).

Main Results:

  • Formation of diverse nanostructures: defect-free PbTe nanocrystals with/without oxide shells, crystalline-Au core/amorphous shell structures, and unique mushroom-shaped Au domains.
  • Detailed structural and compositional analysis of the obtained nanostructures.
  • Observation of sensitivity of these nanostructures to high-intensity electron beams.

Conclusions:

  • The reaction of PbTe nanocrystals with AuCl(3) offers a versatile route to complex nanostructures.
  • The morphology of the resulting nanostructures is controllable by reaction conditions and starting nanocrystal properties.
  • The electron beam sensitivity of these novel PbTe-Au nanostructures warrants further investigation for potential applications and understanding beam-matter interactions.