Related Experiment Video
Updated: Jun 15, 2026
Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury
Published on: March 21, 2021
Reversed changes of lipoxin A4 and leukotrienes in children with asthma in different severity degree
Sheng-Hua Wu1, Pei-Ling Yin, Yong-Mei Zhang
1Department of Pediatrics, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, People's Republic of China.
Objectives:
To investigate the expressions of 15-lipoxygenase (15-LO) and 5-lipoxygenase (5-LO) in leukocytes and the changes of blood lipoxin A(4)(LXA(4)), leukotriene (LT)B(4) and LTC(4) in children with asthma, and to explore the relationship between the blood eicosanoids and one of serum high-sensitivity C-reactive protein (hsCRP), interleukin (IL)-5, IL-8 and IL-13 and IgE in children with asthma.
Study Design:
One hundred six asthmatic children were divided into three groups, that is, mild persistent asthmatic group, moderate persistent asthmatic group and severe persistent asthmatic group. Forty healthy children were served as controls.
Methodology:
The expressions of 15-LO and 5-LO mRNA in leukocytes were assessed by reverse transcription-polymerase chain reaction, and the blood LXA(4), LTB(4), LTC(4), IL-5, IL-8, and IL-13 were determined with enzyme-linked immunosorbent assay. Serum hsCRP was determined with latex-enhanced immuno-turbidimetry kits.
Results:
The leukocytic 15-LO expression and blood LXA(4) were gradually decreased, and the leukocytic 5-LO expression, blood LTB(4), LTC(4), IL-5, IL-8, IL-13, and hsCRP were gradually increased in children with asthma from mild degree to moderate and severe degree. There were positive correlations between blood LXA(4) and one of the peak expiratory flow (PEF) and forced expiratory volume in 1 sec (FEV(1)) percent-predicted values, and negative correlations between blood LTC(4) and one of the PEF and FEV(1) percent-predicted values in children with asthma. There were negative correlations between blood LXA(4) and one of the IL-5, IL-8, IL-13, and hsCRP levels, and positive correlations between one of blood LTB(4), LTC(4) and one of the IL-5, IL-8, IL-13 and hsCRP levels in children with asthma.
Conclusions:
The reversed changes between 15-LO, its product LXA(4) and 5-LO, its products LTB(4) and LTC(4) in children with asthma from mild, moderate to severe degree were found, suggesting that insufficient generation of LXA(4) and overproduction of LTs may be the reason for the asthmatic children whose illness become more serious.
More Related Videos
Related Concept Videos
01:19Antiasthma Drugs: Leukotriene Modifiers
Leukotriene modifiers work through two distinct mechanisms:
01:28Asthma I: Introduction
01:26Asthma-II: Pathophysiology and Classification
Additionally, environmental and genetic factors play crucial roles in determining an individual's susceptibility to asthma and the severity of their condition.
Critical processes in asthma pathophysiology include:
01:13Asthma III: Clinical Manifestations
01:20Asthma: Pathogenesis and Management
Asthma is classified as allergic and non-allergic. Allergens such as dust mites, pollen, and pet dander trigger allergic asthma, while factors like cold air, intense emotions, or exercise can induce non-allergic asthma.
01:25Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs
Mast cell stabilizers, such as cromolyn (also known as sodium cromoglycate) and nedocromil (Tilade), are effective drugs in asthma management. These stabilizers hinder histamine release by skillfully obstructing the activation of mast cells and other cellular entities. Notably, they navigate this task without...