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Related Experiment Video

Updated: Jun 15, 2026

Infinium Assay for Large-scale SNP Genotyping Applications

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

[X-sNP genotyping using the TaqMan probe technology].

Su-hua Zhang¹, Li Li, Cheng-tao Li

¹Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, China. zsh-daisy@163.com

Fa Yi Xue Za Zhi

|March 18, 2010

Summary

This summary is machine-generated.

A new TaqMan probe real-time PCR method accurately detects X chromosome single nucleotide polymorphisms (X-SNPs). This sensitive and rapid technology is suitable for forensic genetics applications.

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Area of Science:

Genetics
Molecular Biology
Forensic Science

Background:

Single nucleotide polymorphisms (SNPs) on the X chromosome are valuable genetic markers.
Accurate and efficient methods for X-SNP genotyping are crucial for various applications.

Purpose of the Study:

To develop a rapid, accurate, and economical real-time fluorescence PCR method for detecting X chromosome single nucleotide polymorphisms (X-SNPs).
To utilize TaqMan probe technology for enhanced specificity and sensitivity in X-SNP detection.

Main Methods:

Design of TaqMan probes and PCR primers targeting 13 X-SNPs.
Genotyping of X-SNPs using real-time fluorescence PCR amplification.
Validation of the developed method against DNA sequencing results.

Main Results:

Successful development of an allele-specific real-time fluorescence PCR assay utilizing TaqMan probes.
The developed method demonstrated high concordance with DNA sequencing for 13 X-SNPs.
All analyzed loci exhibited high polymorphism, with polymorphic information content ranging from 0.3497 to 0.3750 and heterozygosity from 0.4537 to 0.5021.
The loci adhered to Hardy-Weinberg equilibrium.

Conclusions:

The TaqMan probe-based real-time fluorescence PCR is a sensitive, simple, and rapid technology for X-SNP analysis.
The high polymorphism observed in the X-SNP loci suggests their potential utility in forensic genetics.
This method offers a robust tool for genetic studies and forensic investigations involving the X chromosome.