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Related Concept Videos

Nucleosome Remodeling02:54

Nucleosome Remodeling

Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
The Nucleosome01:19

The Nucleosome

Human DNA is almost two meters long. However, it is compressed inside a tiny nucleus measuring only a few microns in diameter. To make this degree of compaction possible, DNA is organized into several sequential levels so that it can fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
In a chromosome, DNA is wound twice around a protein complex called a histone octamer core, which consists of 8 histone proteins. This...
The Nucleosome02:33

The Nucleosome

DNA in a human cell is almost 2m long and it is packed inside a tiny nucleus that is only a few microns in diameter. The level of compaction of DNA inside the nucleus is astonishing. It is organized into several sequentially higher levels of compaction to fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
DNA is wound twice around a protein complex called histone core, that consist of 8 histone proteins. This complex...
The Nucleosome02:33

The Nucleosome

DNA in a human cell is almost 2m long and it is packed inside a tiny nucleus that is only a few microns in diameter. The level of compaction of DNA inside the nucleus is astonishing. It is organized into several sequentially higher levels of compaction to fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
DNA is wound twice around a protein complex called histone core, that consist of 8 histone proteins. This complex...
The Nucleosome Core Particle01:12

The Nucleosome Core Particle

Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...
The Nucleosome Core Particle02:10

The Nucleosome Core Particle

Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...

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Related Experiment Video

Updated: Jun 15, 2026

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells
11:25

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells

Published on: January 25, 2020

DNA architecture, deformability, and nucleosome positioning.

Fei Xu1, Wilma K Olson

  • 1Rutgers, State University of New Jersey, Department of Chemistry and Chemical Biology, BioMaPS Institute for Quantitative Biology, Wright-Rieman Laboratories, 610 Taylor Road, Piscataway, NJ 08854, USA.

Journal of Biomolecular Structure & Dynamics
|March 18, 2010
PubMed
Summary
This summary is machine-generated.

DNA sequence and its shape influence how nucleosomes position on DNA. Understanding these DNA conformational signals aids in predicting nucleosome organization and gene expression.

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Analyzing and Building Nucleic Acid Structures with 3DNA
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Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging

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Last Updated: Jun 15, 2026

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells
11:25

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells

Published on: January 25, 2020

Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging
09:52

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging

Published on: January 31, 2019

Area of Science:

  • Structural biology
  • Genetics
  • Biochemistry

Background:

  • Nucleosome positioning is crucial for DNA organization and gene expression.
  • High-resolution core-particle structures provide insights into DNA-protein interactions.

Purpose of the Study:

  • To investigate DNA conformational signals that dictate nucleosome positioning on specific DNA sequences.
  • To analyze how DNA features in core-particle structures relate to nucleosome placement.

Main Methods:

  • Surveying chemical composition of protein-DNA assemblies.
  • Extracting DNA features like minor-groove width and base-pair deformations from structural data.
  • Utilizing knowledge-based potentials to estimate conformational costs of DNA sequences on nucleosome templates.

Main Results:

  • Identified specific DNA conformational signals within nucleosome core-particle structures.
  • Quantified minor-groove width and base-pair deformations along the DNA superhelical pathway.
  • Assessed the compatibility of crystallized sequences and the '601' sequence with different nucleosome structures.

Conclusions:

  • DNA conformational signals, including minor-groove width and base-pair deformations, play a significant role in nucleosome positioning.
  • Structural analysis of nucleosome core particles reveals sequence-dependent DNA bending and deformation preferences.
  • Knowledge-based potentials can predict the energetic favorability of nucleosome formation on specific DNA sequences.