Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Increased Body Temperature

Increased Body Temperature

A body temperature above 38°C (100.4 °F) is known as fever or pyrexia, and a person with fever is termed 'febrile.' Typically, the hypothalamus, a part of the brain that acts as the body's thermostat, regulates body temperature through a thermoregulatory setpoint. It receives signals from cold and warm thermal receptors throughout the body and adjusts the body's temperature accordingly. Fever occurs when this hypothalamic setpoint is altered, usually in response to an infection or illness.

Decreased Body Temperature

Decreased Body Temperature

A decreased body temperature can occur in patients with hypothermia and frostbite. Heat loss with extended cold exposure overpowers the body's ability to create heat, resulting in hypothermia. Core temperature readings help classify hypothermia. Mild hypothermia is temperatures between 32 °C (89.6 °F) and 35°C (95 °F) and is caused by impaired thermoregulation. Moderate hypothermia is temperatures between 28 C (82.4 °F) and 32 °C (89.6 °F) caused by sustained extreme cold exposure, and severe...

Responses to Heat and Cold Stress

Responses to Heat and Cold Stress

Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.

Physical Methods for Controlling Microbial Growth: Temperature

Physical Methods for Controlling Microbial Growth: Temperature

Heat is a widely used method to control microbial growth by targeting and denaturing cellular proteins, thereby killing or inactivating microbes. This method's effectiveness is quantified using parameters such as the thermal death point (TDP), thermal death time (TDT), and decimal reduction time (D value). TDP represents the lowest temperature at which all microorganisms in a liquid suspension are eliminated within 10 minutes, whereas TDT is the time necessary to achieve sterilization at a...

Factors Influencing Microbial Growth: Temperature

Factors Influencing Microbial Growth: Temperature

Microorganisms display remarkable adaptations, enabling them to thrive in diverse ecological niches across a wide range of temperatures. Temperature profoundly influences microbial growth by affecting enzymatic activity, membrane fluidity, and other cellular processes.Each microorganism operates within a specific temperature range defined by three cardinal points: minimum, optimum, and maximum. Below the minimum temperature, membranes lose fluidity, halting transport processes. Above the...

Requirements for Human Life

Requirements for Human Life

The Earth and its atmosphere have provided humans with air, water, and food, but these are not the only requirements for survival. Humans also require a specific range of temperature and pressure that the Earth and its atmosphere provides.
Oxygen
Atmospheric air is only about 20 percent oxygen, but that oxygen is a key component of the chemical reactions that keep the body alive, including the reactions that produce ATP. Brain cells are susceptible to a lack of oxygen because they require a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A virtual screening and molecular dynamics approach in search of novel antibiotic chemotypes.

PloS one·2026

Same author

Discovery of Sulfonamide Pantothenate Kinase Activators and Elucidation of the Role of Isoform Selectivity in Cellular Pantothenate Kinase Activation.

Journal of medicinal chemistry·2026

Same author

Amplicon sequencing of ice and water phytoplankton and bacterial communities during an extreme winter in a central Canadian great lake.

Microbiology resource announcements·2025

Same author

An open-source screening platform accelerates discovery of drug combinations.

Nature communications·2025

Same author

Mycobacterium tuberculosis overcomes phosphate starvation by extensively remodelling its lipidome with phosphorus-free lipids.

Nature communications·2025

Same author

Prodrug florfenicol amine is activated by intrinsic resistance to target Mycobacterium abscessus.

Nature microbiology·2025

Search research articles

Related Experiment Video

Updated: Jun 15, 2026

High-Throughput Assays of Critical Thermal Limits in Insects

High-Throughput Assays of Critical Thermal Limits in Insects

Published on: June 15, 2020

High temperature pulses decrease indirect chilling injury and elevate ATP levels in the flesh fly, Sarcophaga

Vanessa H Dollo¹, Shu-Xia Yi, Richard E Lee

¹Department of Zoology, Miami University, Oxford, OH 45056, USA.

|March 18, 2010

Summary

Adding brief warming pulses during cold storage significantly improves insect survival by helping flesh flies regenerate energy reserves and reduce chilling injury. This method enhances survival rates during long-term cold exposure.

More Related Videos

Simple Homemade Tools to Handle Fruit Flies—Drosophila melanogaster

Simple Homemade Tools to Handle Fruit Flies—Drosophila melanogaster

Published on: July 24, 2019

Local and Global Methods of Assessing Thermal Nociception in Drosophila Larvae

Local and Global Methods of Assessing Thermal Nociception in Drosophila Larvae

Published on: May 18, 2012

Related Experiment Videos

Last Updated: Jun 15, 2026

High-Throughput Assays of Critical Thermal Limits in Insects

High-Throughput Assays of Critical Thermal Limits in Insects

Published on: June 15, 2020

Simple Homemade Tools to Handle Fruit Flies—Drosophila melanogaster

Simple Homemade Tools to Handle Fruit Flies—Drosophila melanogaster

Published on: July 24, 2019

Local and Global Methods of Assessing Thermal Nociception in Drosophila Larvae

Local and Global Methods of Assessing Thermal Nociception in Drosophila Larvae

Published on: May 18, 2012

Area of Science:

Insect physiology
Cryobiology
Biochemistry

Background:

Indirect chilling injury is a common problem during long-term cold exposure in many organisms, particularly insects.
Previous research indicated that intermittent warming pulses can improve survival and reduce cold injury in flesh flies (Sarcophaga crassipalpis).

Purpose of the Study:

To investigate the impact of a 24-hour warming pulse (15°C or 20°C) on survival rates and adenosine triphosphate (ATP) levels in flesh flies exposed to 0°C.
To determine if warming pulses mitigate indirect chilling injury during prolonged cold storage.

Main Methods:

Flesh flies (Sarcophaga crassipalpis) were subjected to continuous 0°C exposure or a regimen including a 24-hour warming pulse at 15°C or 20°C.
Survival rates were recorded for all experimental groups.
Adenosine triphosphate (ATP) levels were measured in flies from each group.

Main Results:

Flies that experienced a warming pulse exhibited significantly higher survival rates compared to those kept continuously at 0°C.
Adenosine triphosphate (ATP) levels were significantly elevated in flies that underwent a warming pulse, indicating restored energy reserves.
These findings demonstrate a protective effect of warming pulses against cold-induced injury.

Conclusions:

Brief warming pulses during long-term cold storage facilitate the regeneration of energy reserves (ATP) in flesh flies.
Warming pulses are effective in promoting survival and reducing indirect chilling injury in insects under cold conditions.
This strategy offers a potential method for improving the preservation of insects during cold storage.