Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 15, 2026

In vivo Measurement of Knee Extensor Muscle Function in Mice

In vivo Measurement of Knee Extensor Muscle Function in Mice

Published on: March 4, 2021

Functional muscle analysis of the Tcap knockout mouse.

C D Markert¹, M P Meaney, K A Voelker

¹Department of Neurology, Wake Forest University, Winston-Salem, NC 27101, USA.

Human Molecular Genetics

|March 18, 2010

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Taking on the Titin: Muscle imaging as a diagnostic marker of biallelic <i>TTN-</i>related myopathy.

Journal of neuromuscular diseases·2025

Same author

Clinical phenotype of X-linked myotubular myopathy in Labrador Retriever puppies.

Journal of veterinary internal medicine·2015

Same author

Large duplication in MTM1 associated with myotubular myopathy.

Neuromuscular disorders : NMD·2013

Same author

Congenital myopathy caused by a novel missense mutation in the CFL2 gene.

Neuromuscular disorders : NMD·2012

Same author

Nutrition strategies to improve physical capabilities in Duchenne muscular dystrophy.

Physical medicine and rehabilitation clinics of North America·2012

Same author

Acute appendicitis is characterized by a uniform and highly selective pattern of inflammatory gene expression.

Mucosal immunology·2008

Researchers developed a Tcap knockout mouse model for limb-girdle muscular dystrophy type 2G (LGMD2G). These mice exhibit muscle abnormalities and functional deficits, providing a valuable tool for studying LGMD2G and testing therapies.

Area of Science:

Muscle Biology
Genetics
Animal Models

Background:

Autosomal recessive limb-girdle muscular dystrophy type 2G (LGMD2G) is a myopathy affecting skeletal muscle, caused by Tcap gene mutations.
The precise disease mechanisms and a suitable model for study remain elusive.

Purpose of the Study:

To create and characterize a Tcap knockout (KO) mouse model for LGMD2G.
To investigate the effects of Tcap deficiency on skeletal muscle function and histology.

Main Methods:

Generation of Tcap-null mice.
Histological analysis of muscle fibers.
Assessment of myostatin protein levels.
Evaluation of motor function (balance, grip strength) and muscle mechanics.

More Related Videos

In Vivo Electrophysiological Measurement of Compound Muscle Action Potential from the Forelimbs in Mouse Models of Motor Neuron Degeneration

In Vivo Electrophysiological Measurement of Compound Muscle Action Potential from the Forelimbs in Mouse Models of Motor Neuron Degeneration

Published on: June 15, 2018

Related Experiment Videos

Last Updated: Jun 15, 2026

In vivo Measurement of Knee Extensor Muscle Function in Mice

In vivo Measurement of Knee Extensor Muscle Function in Mice

Published on: March 4, 2021

In Vivo Electrophysiological Measurement of Compound Muscle Action Potential from the Forelimbs in Mouse Models of Motor Neuron Degeneration

In Vivo Electrophysiological Measurement of Compound Muscle Action Potential from the Forelimbs in Mouse Models of Motor Neuron Degeneration

Published on: June 15, 2018

Main Results:

Tcap-null mice displayed myofiber size variation and central nucleation, mirroring LGMD2G patient muscle pathology.
Increased myostatin protein levels were observed in Tcap-deficient mice.
KO mice showed impaired balance but no significant differences in muscle force or fatigue resistance.
Increased muscle stiffness was detected in Tcap-null mice.

Conclusions:

The established Tcap KO mouse is a viable model for LGMD2G, exhibiting a comparable dystrophic phenotype.
This model facilitates further mechanistic studies and preclinical therapeutic trials for LGMD2G.