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Updated: Jun 15, 2026

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
Published on: May 18, 2011
Raman and fluorescent scattering by molecules embedded in dielectric spheroids
Abstract:
Fluorescent and Raman scattering by molecules embedded in dielectric particles is strongly dependent on the morphology and optical properties of the particle, the distribution of active molecules within the particle, and, in the case of nonspherical particles, orientation. The model previously applied to spheres and cylinders is now extended to spheroids. The extended boundary condition method (EBCM) has been used to calculate the transmitted field at the incident frequency that stimulates the process. The equivalence principle underlying the EBCM has also been applied to calculate the fields at the shifted frequency. Numerical results are presented to illustrate some of the effects of refractive index, size, shape, and orientation of the particles for models representing two polarizabilities of active dipoles embedded inside the particles.
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