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Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)
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Isopachics measurement using immersion method polarization holography: 2.

T Kihara, H Kubo, R Nagata

    Applied Optics
    |March 18, 2010
    PubMed
    Summary

    This study separates isopachics of birefringent objects using real-time polarization holography and an immersion method. The technique accurately determines the sign of isopachics by observing fringe shifts with a wave front retarder.

    Area of Science:

    • Optics
    • Materials Science
    • Experimental Physics

    Background:

    • Isopachics, representing lines of equal principal stress difference, are crucial for stress analysis in birefringent materials.
    • Previous methods, like moiré patterns, have limitations in separating and determining the sign of isopachics.
    • Real-time polarization holography offers advanced capabilities for optical metrology.

    Purpose of the Study:

    • To develop and demonstrate a novel method for separating isopachics of birefringent objects.
    • To accurately determine the sign of isopachics in real-time.
    • To enhance stress analysis techniques for birefringent materials.

    Main Methods:

    • Utilizing the immersion method in conjunction with real-time polarization holography.

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  • Introducing a wave front retarder into the optical path of the illuminating beam.
  • Observing and analyzing fringe pattern shifts to determine isopachic signs.

    • Main Results:

    • Successful separation of isopachics was achieved using the proposed experimental setup.
    • The sign of the isopachics was accurately determined in real-time.
    • The method demonstrated high precision in identifying stress differences.

    Conclusions:

    • The combined immersion method and real-time polarization holography provide an effective means to separate and sign isopachics.
    • This technique offers a significant advancement for non-destructive stress evaluation in birefringent materials.
    • Accurate real-time stress analysis is achievable with this holographic approach.