Related Experiment Video
Updated: Jun 15, 2026

A Modified QuEChERS-HPLC Method for Detection of Polycyclic Aromatic Hydrocarbons in Zebrafish Embryos Exposed to Fine Particulate Matter
Published on: June 13, 2025
Flow injection solid-phase extraction using multi-walled carbon nanotubes packed micro-column for the determination
Hong Wu1, Xuecui Wang, Bing Liu
1Department of Chemistry, Xuzhou Normal University, Shanghai Road, Xuzhou 221116, China. wuhong1968@hotmail.com
Abstract:
A flow injection solid-phase extraction preconcentration system using a multi-walled carbon nanotubes (MWCNTs) packed micro-column was developed for the determination of 16 polycyclic aromatic hydrocarbons (PAHs) in water by gas chromatography-mass spectrometry (GC-MS). The preconcentration of PAHs on the MWCNTs was carried out based on the adsorption retention of analytes by on-line introducing the sample into the micro-column system. Methanol was introduced to elute the retained analytes for GC-MS analysis using selected ion monitoring (SIM) mode. Important influence factors were studied in detail, such as sample acidity, sample flow rate, eluent flow rate and volume, dimensions of MWCNTs and amounts of packing material. Limits of detection of 16 PAHs for an extraction of 50 mL water sample were in the range of 0.001-0.15 microg L(-1), and the precisions (RSD) were in the range of 4-14%. The optimized method was successfully applied to the determination of 16 PAHs in surface waters, with recoveries in the range of 72-93% for real spiked sample.
Related Concept Videos
Gas Chromatography: Sample Injection Systems
Two primary injection methods are used...
Gas Chromatography: Types of Columns and Stationary Phases
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
Gas Chromatography–Mass Spectrometry (GC–MS)
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall. The coating...
Gas Chromatography: Introduction
In GC, a sample is vaporized and mixed with an inert carrier gas (the mobile phase), which transports it through a column.
High-Performance Liquid Chromatography: Elution Process
Gas Chromatography: Types of Detectors-II

