Triggering of TLR7 and TLR8 expressed by human lung cancer cells induces cell survival and chemoresistance
- 1INSERM U872, Centre de Recherche des Cordeliers, Paris, France.
- 0INSERM U872, Centre de Recherche des Cordeliers, Paris, France.
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View abstract on PubMed
Summary
This summary is machine-generated.Toll-like receptors 7 and 8 (TLR7/TLR8) are expressed by lung tumor cells, promoting their survival and chemoresistance. This suggests TLR signaling directly contributes to cancer development, impacting immunotherapy strategies.
Area Of Science
- Immunology
- Oncology
- Molecular Biology
Background
- Inflammation, cell survival, and cancer are interconnected, with NF-kappaB as a key mediator.
- Toll-like receptors (TLRs) are implicated in tumor development, but TLR7 and TLR8 roles were unconfirmed.
Purpose Of The Study
- To investigate the expression and function of TLR7 and TLR8 in human lung cancer.
- To determine the impact of TLR7 and TLR8 activation on tumor cell survival and chemoresistance.
Main Methods
- Demonstrated TLR7 and TLR8 expression in lung tumor cells (in situ and cell lines).
- Stimulated cells with TLR7/TLR8 agonists and analyzed NF-kappaB activation, Bcl-2 expression, cell survival, and chemoresistance.
- Performed transcriptional analysis on primary lung tumor cells and cell lines.
Main Results
- TLR7 and TLR8 are expressed by human lung tumor cells.
- Activation of TLR7/TLR8 upregulated NF-kappaB and Bcl-2, enhancing tumor cell survival and chemoresistance.
- Gene expression profiles suggested chronic TLR ligand stimulation in tumors.
Conclusions
- TLR7 and TLR8 signaling directly promotes lung tumor development.
- Expression of TLR7/TLR8 in lung tumors should be considered for anticancer immunotherapy development using TLR agonists.
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