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Updated: Jun 15, 2026

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions
Published on: September 30, 2016
Protoplast transformation of filamentous fungi
B Gillian Turgeon1, Bradford Condon, Jinyuan Liu
1Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY, USA.
Abstract:
The protoplast method for the transformation of filamentous fungi is described in detail, as is the Restriction Enzyme-Mediated Integration (REMI) procedure for introducing tagged mutations into the fungal genome. A split marker method for generating PCR fragments for targeted integration and deletion of genes of interest is also detailed.
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