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Tissue factor released from leukemic cells.

T Kubota1, K Andoh, H Sadakata

  • 1Third Department of Internal Medicine, Gunma University School of Medicine, Maebashi, Japan.

Thrombosis and Haemostasis
|January 23, 1991
PubMed
Summary
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Tissue factor (TF) may be released from leukemic cells, correlating with disseminated intravascular coagulation (DIC) development. This study measured TF activity and antigen in acute nonlymphoid leukemia cells and patient plasma.

Area of Science:

  • Hematology
  • Oncology
  • Biochemistry

Background:

  • Tissue factor (TF) is a key initiator of the coagulation cascade.
  • TF expression in acute nonlymphoid leukemia (ANLL) and its association with clinical complications like disseminated intravascular coagulation (DIC) require further investigation.

Purpose of the Study:

  • To investigate the release of TF from HL-60 leukemic cells in vitro.
  • To determine the correlation between TF activity and TF-related antigen in ANLL patients.
  • To assess the potential role of TF in the development of DIC during chemotherapy.

Main Methods:

  • Clotting assays and radioimmunoassay (RIA) were employed to measure TF activity (TFA) and TF-related antigen (TFR:AG).
  • HL-60 cells were cultured with and without endotoxin (EDX) to assess TF release.

Related Experiment Videos

  • TFR:AG levels were measured in ANLL patients' leukemic cells and plasma before and during induction chemotherapy.

    • Main Results:

    • Endotoxin stimulation significantly increased TFA and TFR:AG in HL-60 cell culture medium.
    • A significant positive correlation was observed between TFR:AG and TFA in ANLL patient leukemic cells.
    • TFR:AG levels significantly increased in ANLL patients who developed DIC during chemotherapy, but not in those without DIC.

    Conclusions:

    • TF can be released from leukemic cells into the extracellular environment.
    • Elevated TF levels are associated with ANLL and may play a role in the pathogenesis of DIC in these patients.
    • Monitoring TF may aid in predicting or understanding DIC development in ANLL patients undergoing chemotherapy.