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Ultrastructural localization of Dirofilaria immitis antigen in canine lung tissue using the protein A-gold labelling

J H Tarish1, R B Atwell

  • 1Department of Companion Animal Medicine and Surgery, School of Veterinary Science, University of Queensland, St. Lucia, Australia.

Veterinary Parasitology
|January 1, 1991
PubMed

Dirofilaria immitis antigens were demonstrated in the pulmonary tissues of dogs, with natural and experimental infection (D. immitis) in dogs using an immunostaining colloidal gold technique. Electron microscopy of thin sections treated with anti-heartworm serum revealed antigen deposition within the pulmonary microvasculatur and perivascular areas.

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Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.