Related Experiment Videos
Protein kinase C(19-31) pseudosubstrate inhibition of insulin action in rat adipocytes
M L Standaert1, J Sasse, D R Cooper
1James A. Haley Veterans' Administration Hospital, Tampa, FL 33612.
FEBS Letters
|April 22, 1991
Abstract:
Treatment of intact adipocytes with the autoregulatory PKC pseudosubstrate PKC(19-31) inhibited insulin-stimulated hexose uptake and lipogenesis, with no effect on basal values. The effect was dose-dependent with respective IC50 values of 30 microM and 600 microM for insulin-stimulated hexose uptake in electroporated and intact adipocytes. These studies indicate that PKC may play a role in the mediation of insulin action in adipocytes.