Analysis of some eicosanoids by continuous-flow fast atom bombardment mass spectrometry

Continuous-flow fast atom bombardment has been used to analyze eicosanoids by selected-ion monitoring on a sector-field mass spectrometer operating in the negative-ion mode. The method has been optimized with respect to solvent composition and flow-rates. Detection limits were below 50 pg, and under optimal conditions a linear relationship between response and amount of substance was achieved. The method was successfully applied to the analysis of two spiked urine samples.