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Related Experiment Videos

Tumor antigen presentation by murine epidermal cells.

S Grabbe1, S Bruvers, R L Gallo

  • 1Wellman Laboratories of Photomedicine, Harvard Medical School, Boston, MA 02114.

Journal of Immunology (Baltimore, Md. : 1950)
|May 15, 1991
PubMed
Summary

Murine epidermal cells (EC) pulsed with tumor fragments can induce antitumor immunity. This process requires granulocyte-macrophage-colony-stimulating factor culture and I-A+ cells for effective tumor antigen presentation.

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Area of Science:

  • Immunology
  • Dermatology
  • Oncology

Background:

  • Epidermal Langerhans cells present antigens (Ag) for CD4-dependent immunity.
  • Langerhans cells are hypothesized to present tumor-associated Ag in situ for antitumor immunity.

Purpose of the Study:

  • To investigate the capacity of murine epidermal cells (EC) to present tumor-associated Ag for inducing in vivo antitumor immunity.

Main Methods:

  • Murine EC were depleted of Thy-1+ cells and cultured with granulocyte-macrophage-colony-stimulating factor (GM-CSF).
  • EC were pulsed with tumor fragments (TF) from S1509a-fibrosarcoma and injected into syngeneic recipients.
  • Immunity was assessed by tumor challenge and delayed-type hypersensitivity (DTH) response. I-A+ cells were depleted using antibody and complement lysis.

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Main Results:

  • TF-pulsed EC induced protective immunity against tumor growth in vivo.
  • TF-pulsed EC also induced a significant DTH response to tumor cells.
  • Immunity induction was genetically restricted and required GM-CSF culture.
  • Deletion of I-A+ cells abolished the generation of antitumor immunity.

Conclusions:

  • I-A+ epidermal cells are capable of presenting S1509a tumor Ag.
  • This presentation generates protective antitumor immunity in vivo.
  • GM-CSF culture is essential for the development of this immunity.