Freezing as an aid in the drying and purification of humus and allied materials

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

Sublimation is the direct transformation of a solid to a gaseous state. For instance, at standard pressure and room temperature, solid carbon dioxide sublimes to gaseous carbon dioxide. The phase diagram depicts the conditions required for sublimation. This process occurs at the solid-gas phase boundary and is not observed above the triple point of the substance. The reverse of sublimation is called deposition, where a gaseous substance condenses directly into a solid. Sublimation and...

Concrete's susceptibility to frost damage during freeze-thaw cycles demands strategic measures to enhance its frost resistance. Employing techniques like air entrainment, adjusting the water-cement ratio, proper curing, and selecting appropriate aggregates are essential.
Introducing microscopic air bubbles into the concrete mix through air entrainment creates small voids that accommodate ice expansion, thereby reducing internal pressures and preventing cracking. The optimal amount of entrained...

In cold weather, masonry construction requires specific precautions to ensure mortar does not freeze before curing, as this can significantly weaken its strength and watertightness. Mortar temperature should be maintained between 60°F and 80°F to support proper hydration and curing. Below 40°F, mortar water must be heated, but should not exceed 120°F as high temperatures can reduce mortar's compressive and bond strength.
Other key practices include keeping masonry units and sand dry and...

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...

Concrete structures in cold climates, such as those along roadsides, can retain moisture. This moisture makes them susceptible to frost-related damage when temperatures fall below freezing. Adding moisture worsens the damage during temperature fluctuations, leading to repeated freezing and thawing. De-icing salts, spread over these structures to melt ice, add to the freeze-thaw cycle, and draw even more moisture into the concrete.
This freeze-thaw cycle primarily causes surface scaling, where...