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Updated: Aug 11, 2026

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Production of Replication-Defective Retrovirus by Transient Transfection of 293T cells
Published on: December 4, 2007
Rescue of a transforming virus from a spontaneous nonproducing osteosarcoma in BALB/c mice
Journal of the National Cancer Institute
|February 1, 1978
Abstract:
Cells from spontaneous osteosarcoma V793 that originated in a 19-month-old female BALB/c mouse were cultured. They did not produce a C-type oncovirus as determined by extracellular reverse transcriptase assay and cytoplasmic immunofluorescence. After cocultivation with Balb/3T3 cells chronically infected with a murine leukemia virus (MuLV), a focus-forming principle that transformed 3T3 cells, secondary BALB/c mouse embryo and WAG/Rij rat embryo fibroblasts were rescued. The transformation could be inhibited by antiserum to MuLV.

