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Visualization of hypoxia-induced glutamate release in gerbil hippocampal slice
A Mitani1, F Kadoya, Y Nakamura
1Department of Physiology, School of Medicine, Ehime University, Japan.
Neuroscience Letters
|January 28, 1991
Abstract:
Microfluorometry was used to investigate distribution of hypoxia-induced release of glutamate. Mongolian gerbil hippocampal slice was perfused in a medium containing glutamate dehydrogenase and NAD+. Release of glutamate into extracellular space caused an increase in fluorescence due to the formation of NADH. The hypoxia-induced release of glutamate was gradually increased throughout the slice: no significant difference was detected among CA1 region, CA3 region and the dentate gyrus.