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Mononuclear cell infiltrate and HLA-Dr expression in intra- and extradural secondary carcinomas
M L Rossi1, N R Jones, M M Esiri
1Dept. of Neuropathology, Radcliffe Infirmary, Oxford, Great Britain.
Abstract:
Frozen sections from 30 secondary carcinomas (22 intra- and 8 extradural) were reacted with a panel of monoclonal antibodies to macrophages, lymphocytes, NK cells and HLA-Dr invariant chain. A moderate number of macrophages was demonstrated in 91% of tumours. CD 8 and CD 4 lymphocytes were detected in smaller numbers in 68% and 28% of tumours respectively. B lymphocytes were present in only one tumour and NK cells were absent. There was no significant difference between the mononuclear cell infiltrate in the intra- and extradural tumours and in respect to the histological tumour type. HLA-Dr antigen was expressed by macrophages in most cases and by tumour cells in 5/22 (22%) (4 adeno and 1 anaplastic carcinoma; of which one adenocarcinoma was extradural and the remaining intradural). The results may represent evidence for a degree of cellular immune response to secondary carcinomas which is independent of the intra- or extradural location of the tumour.
Insights
Secondary carcinomas show a cellular immune response, with macrophages present in most tumors. Lymphocytes were also detected, suggesting an immune reaction independent of tumor location.
Area of Science:
- Immunology
- Oncology
- Pathology
Background:
- Secondary carcinomas, particularly those in the dura mater, are complex tumors.
- Understanding the tumor microenvironment and immune cell infiltration is crucial for prognosis.
Purpose of the Study:
- To investigate the presence and type of immune cell infiltrate in secondary carcinomas.
- To determine if tumor location (intradural vs. extradural) or type influences immune cell composition.
Main Methods:
- Frozen sections of 30 secondary carcinomas (22 intradural, 8 extradural) were analyzed.
- Monoclonal antibodies were used to detect macrophages, CD8+ T cells, CD4+ T cells, B cells, NK cells, and HLA-DR invariant chain.
Main Results:
- Macrophages were found in 91% of tumors; CD8+ lymphocytes in 68%; CD4+ lymphocytes in 28%.
- B lymphocytes were rare, and NK cells were absent. No significant differences were observed based on tumor location or type.
- HLA-DR antigen was expressed by macrophages and, in 22% of cases, by tumor cells (adenocarcinomas and anaplastic carcinoma).
Conclusions:
- Secondary carcinomas exhibit a degree of cellular immune response.
- This immune response appears independent of the tumor's intradural or extradural location and histological type.