Immunohistochemical approach to the study of the cat carotid body

The mammalian carotid body contains a number of different cell types which are not always easy to identify in routine histological sections. We have devised a battery of immunohistochemical tests which overcome this difficulty and offer the possibility of performing routine morphometric analyses of the response of the organ to various pathological processes in paraffin-embedded sections. The type 1 cells can be identified on the basis of their reaction with neuronal specific enolase, whilst type II cells react with antibodies to S-100 protein. Schwann cells do not react with S-100 antibodies but do so with antibodies to glial fibrillary acidic protein; nerve fibres can be identified by their reaction to neurofibrillary protein.