Related Experiment Video

Updated: Jun 14, 2026

Sigma's Non-specific Protease Activity Assay - Casein as a Substrate
11:37

Sigma's Non-specific Protease Activity Assay - Casein as a Substrate

Published on: September 17, 2008

The action of tyrosinase on chymotrypsin, trypsin, and pepsin

P EDMAN

    The Journal of Biological Chemistry
    |March 19, 2010
    PubMed
    Abstract

    No abstract available in PubMed .

    Keywords:
    PEPSINTRYPSINTYROSINASE

    More Related Videos

    Qualitative and Quantitative Assays for Detection and Characterization of Protein Antimicrobials10:50

    Qualitative and Quantitative Assays for Detection and Characterization of Protein Antimicrobials

    Published on: April 10, 2016

    Use of the Protease Fluorescent Detection Kit to Determine Protease Activity09:00

    Use of the Protease Fluorescent Detection Kit to Determine Protease Activity

    Published on: August 4, 2009

    Related Concept Videos

    Protein Digestion01:02

    Protein Digestion

    Protein digestion begins in the stomach, where the highly acidic environment can easily disrupt protein structure by exposing the peptide bonds of polypeptide chains. After polypeptide chains are broken into individual amino acids by a series of digestive enzymes, the amino acids are transported to the liver via the bloodstream to produce energy.
    Pancreatic Juice and Secretion01:26

    Pancreatic Juice and Secretion

    Pancreatic juice is a clear fluid produced by the pancreas, containing water, salts, sodium bicarbonate, and enzymes vital for digestion in the small intestine. It helps break down large molecules, facilitating nutrient absorption.
    When acidic chyme from the stomach enters the duodenum, it triggers the release of secretin, a hormone that prompts pancreatic juice secretion. After a fatty meal, cholecystokinin, another hormone, stimulates gallbladder contraction and enhances enzyme-rich...
    Enzymes02:34

    Enzymes

    Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
    Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
    Enzyme-linked Receptors01:00

    Enzyme-linked Receptors

    Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
    Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
    Protein Absorption01:12

    Protein Absorption

    Proteins in the gastrointestinal tract typically come from food, but they can also originate from disintegrated cells or secreted enzymes. In the stomach, the enzyme pepsin breaks down these proteins into polypeptides. The fragments then move into the duodenum as a semi-fluid mass called chyme. Pancreatic proteases, such as trypsin and chymotrypsin, and intestinal brush border enzymes like carboxypeptidases further dismantle the polypeptides into tripeptides, dipeptides, and free amino acids.
    Transducer Mechanism: Enzyme-Linked Receptors01:27

    Transducer Mechanism: Enzyme-Linked Receptors

    Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
    Major types that are helpful drug targets include: