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Cholesterol ester hydrolysis and hormone-sensitive lipase in lactating rat mammary tissue
C A Small1, S J Yeaman, D W West
1Department of Biochemistry and Genetics, University of Newcastle upon Tyne, U.K.
Biochimica Et Biophysica Acta
|April 3, 1991
Abstract:
Neutral cholesterol esterase activity is expressed in extracts of mammary epithelial cells. The identity of the enzyme catalyzing this hydrolysis was investigated. Anti-hormone-sensitive lipase immunoglobulin elicited the total inhibition of this activity and also immunoprecipitated a single phosphoprotein of Mr 84 kDa from mammary cell extracts previously phosphorylated in vitro with [gamma-32P]ATP and cyclic AMP-dependent protein kinase. It is concluded that mammary cell cholesterol esterase activity results from the presence of hormone-sensitive lipase.