Related Experiment Video
Updated: Jun 14, 2026
07:44
An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants
Published on: May 22, 2020
New quantitative electrochemiluminescence method (ECLIA) for interleukin-6 (IL-6) measurement
Belén Prieto1, Diego Miguel, Marta Costa
1Clinical Biochemistry Laboratory, Hospital Universitario Central de Asturias, Oviedo, Spain.
Clinical Chemistry and Laboratory Medicine
|March 20, 2010
Summary
A new quantitative interleukin-6 (IL-6) assay shows good reproducibility for measuring IL-6 in cord blood plasma. This method establishes a new reference limit for IL-6, potentially aiding sepsis diagnosis in newborns.
Area of Science:
- Clinical Chemistry
- Neonatal Medicine
- Immunodiagnostics
Background:
- Evaluation of a novel interleukin-6 (IL-6) electrochemiluminescent immunoassay (ECLIA).
- Comparison with a previous semiquantitative immunoassay for IL-6.
- Establishment of reference ranges for IL-6 in neonatal cord blood plasma.
Purpose of the Study:
- To assess the performance of a new quantitative IL-6 ECLIA.
- To compare the ECLIA method with an existing semiquantitative immunoassay.
- To determine reference limits for IL-6 in cord blood plasma.
Main Methods:
- IL-6 ECLIA performed on a Cobas E601 analyzer.
- Comparison using a semiquantitative lateral flow immunoassay with digital image capture.
- Recruitment of healthy newborns to establish cord blood plasma IL-6 reference ranges.
Main Results:
- Total imprecision for IL-6 ECLIA ranged from 3.7% to 8.0%.
- The limit of detection for IL-6 ECLIA was 2.63 pg/mL.
- A reference limit of 30 pg/mL for IL-6 in cord blood plasma was established from 148 samples.
Conclusions:
- The new quantitative IL-6 ECLIA method demonstrates excellent reproducibility, linearity, and sensitivity.
- The established IL-6 reference limit in cord blood plasma is novel.
- Further clinical studies are required to validate the utility of this IL-6 reference limit for diagnosing vertical transmission of sepsis.
