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Updated: Jun 14, 2026

DNA-barcode-based Multiplex Immunofluorescence Imaging to Analyze FFPE Specimens from Genetically Reprogrammed Murine Melanoma
Published on: June 6, 2025
Size functions for the morphological analysis of melanocytic lesions
Massimo Ferri1, Ignazio Stanganelli
1Department of Mathematics, The Advanced Research Center on Electronic Systems for Information and Communication Technologies E. De Castro (ARCES), University of Bologna, 40126 Bologna, Italy. ferri@dm.unibo.it
Abstract:
Size Functions and Support Vector Machines are used to implement a new automatic classifier of melanocytic lesions. This is mainly based on a qualitative assessment of asymmetry, performed by halving images by several lines through the center of mass, and comparing the two halves in terms of color, mass distribution, and boundary. The program is used, at clinical level, with two thresholds, so that comparison of the two outputs produces a report of low-middle-high risk. Experimental results on 977 images, with cross-validation, are reported.
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