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Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

A microfluidic chip-compatible bioassay based on single-molecule detection with high sensitivity and multiplexing.

Randall E Burton1, Eric J White, Ted R Foss

  • 1U. S. Genomics, Inc., 12 Gill St., Suite 4700, Woburn, MA 01801, USA. rburton@usgenomics.com

Lab on a Chip
|March 20, 2010
PubMed
Summary

Researchers developed Digital DNA sensors for highly multiplexed detection in microfluidics. These tiny, biocompatible sensors overcome clogging issues, enabling simultaneous analysis of multiple targets in minimal volumes.

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Last Updated: Jun 14, 2026

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Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Molecular Diagnostics

Background:

  • Multiplexed detection is crucial for pharmaceutical development, diagnostics, and research.
  • Current suspension arrays use 5-micrometer beads incompatible with microfluidics due to clogging.
  • A need exists for miniaturized, high-information-content, low-cost detection particles.

Purpose of the Study:

  • To design and demonstrate a novel DNA-based sensor for multiplexed detection compatible with microfluidic devices.
  • To overcome the limitations of existing bead-based suspension arrays.
  • To create a versatile platform for simultaneous identification and quantification of various analytes.

Main Methods:

  • Utilized specialized recombinant DNA (Digital DNA) as the core detection particle.
  • Engineered Digital DNA to contain analyte-recognition units and a geometric identification pattern.
  • Fabricated Digital DNA nanoparticles with a size compatible with 1-micrometer deep microfluidic chips.

Main Results:

  • The Digital DNA sensor achieved high multiplexing capability and high sensitivity.
  • Demonstrated biocompatibility of the DNA-based sensor particles.
  • Confirmed particle size compatibility with microfluidic systems, preventing clogging.

Conclusions:

  • Digital DNA nanoparticles offer a promising solution for miniaturized, multiplexed sensing.
  • This technology enables simultaneous detection of proteins, nucleic acids, viruses, and toxins in minimal volumes.
  • The Digital DNA sensor platform is expected to be broadly applicable across various research and diagnostic fields.