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A novel nitric oxide producing probiotic patch and its antimicrobial efficacy: preparation and in vitro analysis

Mitchell Lawrence Jones1, Jorge G Ganopolsky, Alain Labbé

  • 1Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering and Physiology, Artificial Cells and Organs Research Centre, Faculty of Medicine McGill University, 3775 University Street, Montreal, QC, Canada. mitchell.jones@mcgill.ca

Applied Microbiology and Biotechnology
|March 20, 2010
PubMed

Insights

A novel probiotic patch effectively kills wound pathogens by producing nitric oxide (NO). This innovative antimicrobial wound care solution demonstrates significant efficacy against common bacterial and fungal infections in vitro.

Area of Science:

  • Biotechnology
  • Microbiology
  • Wound Healing

Background:

  • Microbial and fungal infections are a major cause of wound complications.
  • Existing antimicrobial and antifungal treatments have variable efficacy and stability.
  • Novel strategies are needed for effective wound infection management.

Purpose of the Study:

  • To develop and evaluate a nitric oxide (NO)-producing probiotic adhesive patch for antimicrobial and antifungal applications.
  • To investigate the in vitro efficacy of the probiotic patch against common wound pathogens.

Main Methods:

  • Immobilized lactic acid bacteria, glucose, and nitrite salts were used to generate gaseous nitric oxide (gNO).
  • The gNO-producing probiotic patch was applied to cultures of various bacterial and fungal pathogens.
  • Antimicrobial and antifungal efficacy was assessed by monitoring pathogen survival over time.

Main Results:

  • The gNO-producing probiotic patch achieved complete cell death for E. coli, S. aureus, P. aeruginosa, MRSA, T. mentagrophytes, and T. rubrum within 4-8 hours.
  • Significant reduction in A. baumannii colonies (<10 CFU/mL) was observed at 6 hours.
  • The patch demonstrated sustained gNO production over a therapeutically relevant duration.

Conclusions:

  • A gNO-producing probiotic patch effectively eliminates common bacterial and fungal wound pathogens in vitro.
  • This device offers a promising new approach for antimicrobial wound care.
  • The patch utilizes a probiotic mechanism for sustained gNO release and pathogen eradication.

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