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Related Experiment Video

Updated: Jun 14, 2026

A Multi-Parametric Islet Perifusion System within a Microfluidic Perifusion Device
07:55

A Multi-Parametric Islet Perifusion System within a Microfluidic Perifusion Device

Published on: January 26, 2010

Microfluidic perifusion and imaging device for multi-parametric islet function assessment.

Adeola F Adewola1, Dongyoung Lee, Tricia Harvat

  • 1Department of Transplant/Surgery, University of Illinois, Chicago, IL, USA.

Biomedical Microdevices
|March 20, 2010
PubMed
Summary

A novel microfluidic device enables dynamic assessment of pancreatic islet function by simultaneously imaging insulin secretion, calcium influx, and mitochondrial changes. This tool aids in islet research and transplantation readiness.

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Area of Science:

  • Biomedical Engineering
  • Endocrinology
  • Cell Biology

Background:

  • Pancreatic islets are crucial for glucose homeostasis and insulin secretion.
  • Assessing islet function dynamically requires advanced microfluidic tools.
  • Simultaneous monitoring of cellular responses provides deeper insights.

Purpose of the Study:

  • To develop and validate a microfluidic islet perifusion device for dynamic functional assessment.
  • To enable simultaneous fluorescence imaging of insulin secretion, calcium influx, and mitochondrial potential.
  • To provide a tool for islet quality control and in vitro functional studies.

Main Methods:

  • Development of a second-generation microfluidic islet perifusion device with optimized fluid dynamics.
  • Perifusion of multiple pancreatic islets with controlled glucose gradients.

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Imaging Calcium Dynamics in Subpopulations of Mouse Pancreatic Islet Cells
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Last Updated: Jun 14, 2026

A Multi-Parametric Islet Perifusion System within a Microfluidic Perifusion Device
07:55

A Multi-Parametric Islet Perifusion System within a Microfluidic Perifusion Device

Published on: January 26, 2010

Human Pseudoislet System for Synchronous Assessment of Fluorescent Biosensor Dynamics and Hormone Secretory Profiles
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Human Pseudoislet System for Synchronous Assessment of Fluorescent Biosensor Dynamics and Hormone Secretory Profiles

Published on: November 3, 2023

Imaging Calcium Dynamics in Subpopulations of Mouse Pancreatic Islet Cells
08:03

Imaging Calcium Dynamics in Subpopulations of Mouse Pancreatic Islet Cells

Published on: November 26, 2019

  • Simultaneous fluorescence imaging of intracellular calcium and mitochondrial membrane potential.

    • Main Results:

    • The device achieved efficient mixing and uniform solution distribution for reproducible glucose gradients.
    • High signal-to-noise ratio and spatial-temporal resolution were obtained for simultaneous imaging.
    • The system successfully monitored dynamic changes in islet function in response to glucose stimulation.

    Conclusions:

    • The developed microfluidic system is effective for detailed studies of pancreatic islet endocrine function.
    • It allows for simultaneous imaging of intracellular ion fluxes and mitochondrial membrane potential.
    • This technology serves as a valuable tool for islet preparation quality assessment and in vitro functional analysis.