Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 14, 2026

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems
08:17

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Published on: July 4, 2011

[DNA nanosensor fluorescence imaging microscopy].

Chu-yun Deng1, Jia-min Li, Wan-yun Ma

  • 1The Key Laboratory of Atomic and Molecular Nanosciences, Ministry of Education, Department of Physics, Tsinghua University, Beijing 100084, China. dcy04@mails.tsinghua.edu.cn

Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu
|March 23, 2010
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to "A new perspective on idiopathic pulmonary fibrosis research: From multi-dimensional mechanistic exploration to advances in precision therapy" [Respir. Med. 260 (2026) 1-15].

Respiratory medicine·2026
Same author

A new perspective on idiopathic pulmonary fibrosis research: From multi-dimensional mechanistic exploration to advances in precision therapy.

Respiratory medicine·2026
Same author

CAR-Cell Therapy for Autoimmune Diseases: From the Laboratory to Clinical Practice.

Journal of immunology research·2026
Same author

Luteolin Improves BBB Integrity Following Ischemic Stroke Injury by Downregulating the Expression of LRP1 and MMP9.

Phytotherapy research : PTR·2026
Same author

Hybrid prodrug-nanoassemblies coated by chitosan exerted antioxidant and anti-inflammatory effect on liver fibrosis.

International journal of biological macromolecules·2026
Same author

Will Teacher-AI Collaboration Enhance Teaching Engagement?

Behavioral sciences (Basel, Switzerland)·2025
Same journal

The Laser Rangefinder System in Quadrature Modem and Ambiguity Resolution.

Guang pu xue yu guang pu fen xi = Guang pu·2018
Same journal

Improving the Accuracy of Camera-Based Heart Rate Measurement.

Guang pu xue yu guang pu fen xi = Guang pu·2018
Same journal

Determination of Pb, Cr, Cd, and As in Aluminum-Plastic Packaging Materials via Inductively Coupled Plasma-Mass Spectrometry with Microwave Digestion.

Guang pu xue yu guang pu fen xi = Guang pu·2018
Same journal

Study on Molecular Recognition of Cucurbit[6]uril with Oxytetracycline Molecules by Spectroscopic Methods.

Guang pu xue yu guang pu fen xi = Guang pu·2018
Same journal

Preparation and Properties of Novel Polymer Blue Fluorescent Materials.

Guang pu xue yu guang pu fen xi = Guang pu·2018
Same journal

Effect of the Nitrogen Incorporation on the Microstructure and Photoelectric Properties of N Type Nanocrystalline Silicon Thin Films.

Guang pu xue yu guang pu fen xi = Guang pu·2018
See all related articles

This study demonstrates a novel quantum dot DNA nanosensor for detecting DNA/RNA segments in living cells. The sensor utilizes fluorescence resonance energy transfer (FRET) for sensitive and real-time monitoring of target sequences.

Area of Science:

  • Nanotechnology
  • Biophysics
  • Molecular Biology

Context:

  • Quantum dots (QDs) offer superior optical properties, making them ideal for fluorescence-based detection.
  • Existing fluorescence probes face limitations in sensitivity and real-time cellular imaging.
  • Developing advanced nanosensors is crucial for precise biomolecule detection in complex biological environments.

Purpose:

  • To design and validate a quantum-dot-based DNA nanosensor for detecting DNA or RNA segments.
  • To investigate the sensor's capability for real-time fluorescence resonance energy transfer (FRET) imaging.
  • To demonstrate the nanosensor's application in detecting nucleic acid targets within living cells.

Summary:

  • A DNA nanosensor was constructed using streptavidin-conjugated QDs and biotinylated reporter probes (Cy5).

More Related Videos

Fluorescence Imaging with One-nanometer Accuracy (FIONA)
11:56

Fluorescence Imaging with One-nanometer Accuracy (FIONA)

Published on: September 26, 2014

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
14:36

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

Related Experiment Videos

Last Updated: Jun 14, 2026

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems
08:17

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Published on: July 4, 2011

Fluorescence Imaging with One-nanometer Accuracy (FIONA)
11:56

Fluorescence Imaging with One-nanometer Accuracy (FIONA)

Published on: September 26, 2014

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
14:36

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

  • The sensor detects target DNA/RNA via FRET between QDs and Cy5, visualized using ICCD fluorescence microscopy and TIRF.
  • The study successfully detected 30-base ssDNA in solution and demonstrated intracellular FRET upon QD and Cy5-ssDNA-Biotin uptake by cells.

    • Impact:

    • Establishes the feasibility of using QD-based nanosensors for intracellular DNA/RNA detection.
    • Provides a powerful tool for real-time monitoring of nucleic acid interactions in live cells.
    • Advances the field of molecular diagnostics and cellular imaging with enhanced sensitivity and specificity.