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One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode
Published on: June 18, 2016
Helicobacter pylori cagA gene polymorphism affects the total antioxidant capacity of human saliva
Maciej Wnuk1, Aleksander Myszka, Anna Lewinska
1Department of Genetics, University of Rzeszow, Rzeszow, Poland. mawnuk@gmail.com
Background:
We aimed to evaluate the total antioxidant capacity (TAC) of saliva in healthy Helicobacter pylori-positive and negative saliva individuals.
Materials And Methods:
A total of 102 human saliva samples were checked for the presence of H. pylori DNA (ureA and cagA gene fragments). TAC of saliva was estimated by ABTS radical cation (ABTS( +)) decolorization assay.
Results:
PCR analysis revealed that 36 subjects were ureA-/cagA-, 24 were ureA+/cagA- and 42 were ureA+/cagA+. Smoking habits had no evident effect on H. pylori infection. We found that TAC of the ureA-/cagA- material, after 10 seconds reaction reflecting fast-reacting antioxidants, was significantly higher than of ureA+/cagA- and ureA+/cagA+ samples (p < .01 and p < .001, respectively). Similar results were obtained for reaction time of 3 minutes measuring slow-reacting antioxidants (p < .001). We also estimated ureA+/cagA- and ureA+/cagA+ samples alone and reported a statistically significant decrease in the TAC(3 min) value of ureA+/cagA+ compared with ureA+/cagA- samples (p < .05).
Conclusions:
Our data demonstrated that altered redox equilibrium may be associated with more frequent occurrence of H. pylori in the saliva samples.
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