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Functionalised ZnO-nanorod-based selective electrochemical sensor for intracellular glucose
Muhammad H Asif1, Syed M Usman Ali, Omer Nur
1Department of Science and Technology, Campus Norrköping, Linköping University, Bredgatan 34, SE-601 74 Norrköping, Sweden. muhas@itn.liu.se
Biosensors & Bioelectronics
|March 23, 2010
Summary
This study developed a novel ZnO nanorod sensor for measuring intracellular glucose. The sensor accurately quantified glucose in cells and demonstrated insulin
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Electrochemistry
Background:
- Accurate measurement of intracellular glucose is crucial for understanding cellular metabolism and disease.
- Existing methods for intracellular glucose detection are often complex or invasive.
Purpose of the Study:
- To develop a selective electrochemical sensor for intracellular glucose detection.
- To validate the sensor's performance in biological samples and its response to insulin.
Main Methods:
- Fabrication of a sensor using hexagonal zinc oxide (ZnO) nanorods grown on a glass capillary tip.
- Coating ZnO nanorods with glucose oxidase enzyme.
- Electrochemical measurement of potential difference against an Ag/AgCl reference microelectrode.
- Testing the sensor in human adipocytes and frog oocytes.
Main Results:
- The ZnO nanorod sensor demonstrated a glucose-dependent electrochemical potential difference.
- The sensor showed a linear response over a physiologically relevant glucose concentration range (0.5–1000 µM).
- Intracellular glucose measurements in adipocytes and oocytes correlated with literature values.
- The sensor successfully detected insulin-induced changes in intracellular glucose levels.
Conclusions:
- A functionalized ZnO nanorod-based electrochemical sensor provides a simple and effective method for intracellular glucose measurement.
- This nanoelectrode device offers a valuable tool for studying cellular glucose dynamics and the effects of hormones like insulin.
