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Cyclic adenosine monophosphate production by embryonic chick cells
Science (New York, N.Y.)
|March 3, 1978
Summary
Chick embryo cells release cyclic adenosine monophosphate (cyclic AMP) when stimulated. This response has specific concentration thresholds and occurs rapidly, with cells releasing millions of molecules per pulse.
Area of Science:
- Cellular biology
- Biochemistry
Background:
- Cellular communication relies on signaling molecules.
- Cyclic adenosine monophosphate (cyclic AMP) is a key second messenger.
Purpose of the Study:
- To investigate the cellular response of chick embryo cells to cyclic AMP stimulation.
- To determine the dose-response characteristics of cyclic AMP-induced signaling.
Main Methods:
- Dissociation of cells from 1-day-old chick embryos.
- Stimulation of cells with varying concentrations of cyclic AMP.
- Measurement of cyclic AMP pulse production.
Main Results:
- Cells produced a pulse of cyclic AMP upon stimulation.
- A threshold concentration of approximately 10(-8) M was observed.
- An upper limit for response suppression was found around 6 X 10(-6) M.
- The response initiated within 5 seconds, with an average release of 10(7) molecules per cell.
Conclusions:
- Chick embryo cells exhibit a rapid and dose-dependent cyclic AMP signaling response.
- The findings provide insights into early cellular signaling mechanisms.