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A Multiplex Serological Assay for the Detection of Antibody Responses to Arboviruses
Published on: November 4, 2025
Development of a rapid and comprehensive proteomics-based arboviruses detection system
Kenta Okamoto1, Yushirou Endo, Shingo Inoue
1Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan.
Journal of Virological Methods
|March 23, 2010
Summary
A new protocol rapidly identifies arboviruses, including rare types like Banna virus, using liquid chromatography-electrospray ionisation-tandem mass spectrometry. This method offers a faster alternative to traditional techniques for viral screening.
Area of Science:
- Virology
- Analytical Chemistry
- Biotechnology
Background:
- Arboviruses pose significant public health challenges.
- Accurate and rapid identification of arboviruses is crucial for disease control.
- Conventional methods for arbovirus detection can be time-consuming and may fail to identify uncommon strains.
Purpose of the Study:
- To develop a rapid and comprehensive protocol for arbovirus identification.
- To establish a method combining simple purification with liquid chromatography-electrospray ionisation-tandem mass spectrometry (LC-ESI/MS/MS).
- To enable the detection of a wide range of arboviruses, including previously difficult-to-identify ones.
Main Methods:
- A protocol integrating simple purification techniques.
- Application of liquid chromatography-electrospray ionisation-tandem mass spectrometry (LC-ESI/MS/MS).
- Testing the protocol on infected culture fluid samples.
Main Results:
- Successful identification of various arboviruses.
- Detection of uncommon arboviruses, such as Banna virus and Yunnan orbivirus.
- Demonstration of the protocol's effectiveness in identifying viruses missed by conventional methods.
Conclusions:
- The developed LC-ESI/MS/MS protocol provides a rapid and comprehensive method for arbovirus identification.
- This approach is valuable for screening viral samples that are challenging for conventional gene amplification or immunological assays.
- The protocol facilitates the detection of both common and uncommon arboviruses, enhancing diagnostic capabilities.
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