Related Experiment Video
Updated: Jun 14, 2026

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock
Published on: June 29, 2017
Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) from unrelated carbon sources by metabolically engineered
Zheng-Jun Li1, Zhen-Yu Shi, Jia Jian
1Department of Biology, School of Life Sciences, Tsinghua University, Haidian District, Beijing 100084, China.
Abstract:
A metabolically engineered Escherichia coli has been constructed for the production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] from unrelated carbon sources. Genes involved in succinate degradation in Clostridium kluyveri and P(3HB) accumulation pathway of Ralstonia eutropha were co-expressed for the synthesis of the above copolyester. E. coli native succinate semialdehyde dehydrogenase genes sad and gabD were both deleted for eliminating succinate formation from succinate semialdehyde, which functioned to enhance the carbon flux to 4HB biosynthesis. The metabolically engineered E. coli produced 9.4 gl(-1) cell dry weight containing 65.5% P(3HB-co-11.1 mol% 4HB) using glucose as carbon source in a 48 h shake flask growth. The presence of 1.5-2 gl(-1) alpha-ketoglutarate or 1.0 gl(-1) citrate enhanced the 4HB monomer content from 11.1% to more than 20%. In a 6l fermentor study, a 23.5 gl(-1) cell dry weight containing 62.7% P(3HB-co-12.5 mol% 4HB) was obtained after 29 h of cultivation. To the best of our knowledge, this study reports the highest 4HB monomer content in P(3HB-co-4HB) produced from unrelated carbon sources.
More Related Videos
Related Concept Videos
Production of Pharmaceuticals
Bioplastics
Production of Organic Acids
Bioreactor Controls-III
Production of Antibiotics
Bioremediation

