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Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
Published on: April 16, 2015
Naive CD4+ T lymphocytes circulate through lymphoid organs to interact with endogenous antigens and upregulate their
Michio Tomura1, Kikuji Itoh, Osami Kanagawa
1Laboratory for Autoimmune Regulation, Research Center for Allergy and Immunology, RIKEN Yokohama Institute, Yokohama City, Japan.
Journal of Immunology (Baltimore, Md. : 1950)
|March 23, 2010
Summary
Naive T cells interact with antigens in lymphoid organs, increasing CD69 expression and enhancing cytokine production. This interaction is crucial for T cell reactivity and immune responses.
Area of Science:
- Immunology
- Cellular Biology
Background:
- Naive T lymphocytes continuously recirculate through the lymph-vascular system.
- These cells enter and exit lymphoid organs as part of their surveillance function.
Purpose of the Study:
- To investigate the interaction of naive T cells with endogenous antigens within lymphoid organs.
- To understand the role of CD69 expression in T cell behavior and function.
Main Methods:
- Utilized mice expressing the photoconvertible fluorescence protein Kaede for cell tracking.
- Monitored naive T cell migration, CD69 expression, and cytokine production in response to antigen interaction.
Main Results:
- Naive T cells interact with endogenous antigens in lymphoid organs, inducing transient CD69 expression.
- CD69 expression prolongs T cell retention in lymphoid organs; lack of expression leads to migration.
- CD69-positive naive CD4(+) T cells show enhanced and accelerated cytokine production compared to CD69-negative cells.
Conclusions:
- Naive CD4(+) T cells continuously migrate to interact with endogenous antigens.
- This antigen interaction is vital for modulating the reactivity and function of naive CD4(+) T cells.
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