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Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids
Published on: May 12, 2023
Bioorthogonal turn-on probes for imaging small molecules inside living cells
Neal K Devaraj1, Scott Hilderbrand, Rabi Upadhyay
1Center for Systems Biology, Massachusetts General Hospital, Boston, 02114, USA.
Angewandte Chemie (International Ed. in English)
|March 23, 2010
Abstract
None:
Glowing tags: a series of activatable ("turn-on") tetrazine-conjugated fluorescent probes was developed, which react rapidly in an inverse-electron-demand [4+2] cycloaddition with strained dienophiles such as trans-cyclooctene, thereby strongly increasing the fluorescence intensity. The novel turn-on probes were applied for intracellular live-cell imaging of a microtubuli-binding trans-cyclooctene modified taxol.

