Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Polymorphisms in intron 1 of HLA-DRA differentially associate with type 1 diabetes and celiac disease and implicate involvement of complement system genes C4A and C4B.

eLife·2026

Same author

Etiologic Determinants and Characteristics of Diabetes in Haitian Youth (EDDHY Study).

Pediatric diabetes·2025

Same author

Fifty years of HLA-associated type 1 diabetes risk: history, current knowledge, and future directions.

Frontiers in immunology·2024

Same author

NKp44/HLA-DP-dependent regulation of CD8 effector T cells by NK cells.

Cell reports·2024

Same author

Complete HLA genotyping of type 1 diabetes patients and controls from Mali reveals both expected and novel disease associations.

HLA·2024

Same author

Targeting hepatitis B vaccine escape using immunogenetics in Bangladeshi infants.

medRxiv : the preprint server for health sciences·2023

Search research articles

Related Experiment Video

Updated: Jun 14, 2026

Dried Blood and Serum Spots As A Useful Tool for Sample Storage to Evaluate Cancer Biomarkers

Dried Blood and Serum Spots As A Useful Tool for Sample Storage to Evaluate Cancer Biomarkers

Published on: June 11, 2018

Maximizing deoxyribonucleic acid yield from dried blood spots.

Julie A Lane¹, Janelle A Noble

¹Children's Hospital Oakland Research Institute, Oakland, California 94609 , USA.

Journal of Diabetes Science and Technology

|March 24, 2010

Summary

Maximizing deoxyribonucleic acid (DNA) yield from dried blood spots is crucial for genetic studies. Modified extraction protocols significantly increased DNA recovery, enabling successful downstream applications like polymerase chain reaction.

More Related Videos

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Published on: March 13, 2015

Dried Blood Spot Collection of Health Biomarkers to Maximize Participation in Population Studies

Dried Blood Spot Collection of Health Biomarkers to Maximize Participation in Population Studies

Published on: January 28, 2014

Related Experiment Videos

Last Updated: Jun 14, 2026

Dried Blood and Serum Spots As A Useful Tool for Sample Storage to Evaluate Cancer Biomarkers

Dried Blood and Serum Spots As A Useful Tool for Sample Storage to Evaluate Cancer Biomarkers

Published on: June 11, 2018

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Published on: March 13, 2015

Dried Blood Spot Collection of Health Biomarkers to Maximize Participation in Population Studies

Dried Blood Spot Collection of Health Biomarkers to Maximize Participation in Population Studies

Published on: January 28, 2014

Area of Science:

Genetics
Molecular Biology
Biochemistry

Background:

Dried blood spots (Guthrie cards) are a valuable resource for genetic studies.
DNA extraction from these cards can result in variable yields.
Optimizing DNA recovery from this nonrenewable resource is essential.

Purpose of the Study:

To maximize deoxyribonucleic acid (DNA) yield from dried blood spots.
To improve DNA extraction efficiency from Guthrie cards.

Main Methods:

Utilized commercial DNA extraction kits.
Introduced protocol modifications, including a pre-extraction solvent step.
Optimized the elution process with prewarmed buffer and elevated temperature soaking.

Main Results:

A commercial solvent enhanced DNA yields compared to standard extraction.
Modified elution steps increased DNA yield by 6- to 10-fold.
Achieved up to 660 ng of DNA from a single 5-mm blood spot punch.

Conclusions:

The modified DNA extraction method significantly improves yield from dried blood spots.
The extracted DNA is suitable for downstream genetic analyses, including polymerase chain reaction (PCR).