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Updated: Jun 14, 2026

Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice

Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice

Published on: March 11, 2020

Imaging extrasynaptic glutamate dynamics in the brain.

Yohei Okubo¹, Hiroshi Sekiya, Shigeyuki Namiki

¹Departments of Pharmacology and Neurobiology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.

Proceedings of the National Academy of Sciences of the United States of America

|March 24, 2010

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Synaptic activity creates glutamate dynamics outside of synapses, activating receptors and showing crosstalk between them. This study visualizes these dynamics in live animals, revealing spatiotemporal features.

Area of Science:

Neuroscience
Neurobiology
Molecular Biology

Background:

Glutamate is the primary excitatory neurotransmitter in the brain.
It mediates transmission across the synaptic cleft in excitatory synapses.

Purpose of the Study:

To investigate extrasynaptic glutamate dynamics.
To visualize synaptic activity in live animals.

Main Methods:

Utilized a novel glutamate imaging method with fluorescent indicators.
Observed glutamate dynamics in brain slices and in vivo.

Main Results:

Synaptic activity generates measurable extrasynaptic glutamate dynamics.
These dynamics activate extrasynaptic glutamate receptors.

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Crosstalk between neighboring synapses was observed.

Sensory input induced extrasynaptic glutamate dynamics in the cerebral cortex in vivo.

Conclusions:

The study clarifies the spatiotemporal features of extrasynaptic glutamate dynamics.
Provides a new method for directly visualizing synaptic activity in live animals.