Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cross-reactivity00:42

Cross-reactivity

Overview

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neuroprotective efficacy of Curcumae Rhizoma against ischemic stroke via attenuation of COX-2-mediated neuroinflammation: a systematic study predicting thrombin inhibition and highlighting alexandrin and hederagenin.

Metabolic brain disease·2026
Same author

Implementation of an AI-Based Clinical Decision Support System Predicting In-Hospital Cardiac Arrest in General Wards: A Multicenter Staggered-Implementation Study in Secondary Hospitals in Korea.

Diagnostics (Basel, Switzerland)·2026
Same author

Highly Flexible and Conformable ZnO/FeGa Magnetoelectric Heterostructures for Skin wound Healing.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Effect of Optical Coherence Tomography Angiography Image Artifacts on Quantitation of Vessel Density and Perfusion.

Ophthalmic surgery, lasers & imaging retina·2026
Same author

Correction: Solar-powered bioelectrochemical system for efficient cadmium remediation and recovery of reusable solids.

RSC advances·2026
Same author

Risk of Immunologic Reactions During Monthly Therapy for Hansen Disease.

JAMA dermatology·2026

Related Experiment Video

Updated: Jun 14, 2026

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
10:17

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

Combinatorial libraries against libraries for selecting neoepitope activation-specific antibodies.

Xuebo Hu1, Sungkwon Kang, Craig Lefort

  • 1Department of Biomedical Engineering, Cornell University, Ithaca, NY 14853, USA.

Proceedings of the National Academy of Sciences of the United States of America
|March 24, 2010
PubMed
Summary

Researchers developed a novel method to discover conformation-specific antibodies, successfully isolating potent antagonists for activated integrin Mac-1 (αMβ2) that inhibit neutrophil adhesion and migration.

More Related Videos

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
12:55

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein
08:04

Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein

Published on: December 20, 2017

Related Experiment Videos

Last Updated: Jun 14, 2026

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
10:17

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
12:55

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein
08:04

Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein

Published on: December 20, 2017

Area of Science:

  • Immunology
  • Molecular Biology
  • Biochemistry

Background:

  • Conformation-specific antibodies are crucial for understanding cellular signaling and developing targeted therapies.
  • Integrin Mac-1 (αMβ2) plays a key role in neutrophil adhesion and migration, making it a target for therapeutic intervention.

Purpose of the Study:

  • To develop a systematic strategy for isolating conformation-specific antibodies targeting activated integrin Mac-1 (αMβ2).
  • To generate potent antibody antagonists capable of blocking neutrophil adhesion and migration.

Main Methods:

  • Yeast surface display of a Mac-1 inserted (I) domain library.
  • Directed evolution to isolate active I domain mutants.
  • Phage display screening against the active I domain.
  • Yeast surface two-hybrid system for quantitative selection of antibodies.

Main Results:

  • Efficient isolation of intermediate to high affinity antibodies against the active Mac-1 I domain.
  • Demonstrated that isolated antibodies antagonize I domain binding to ICAM-1, iC3b, and fibronectin.
  • Showcased potent inhibition of neutrophil migration on fibrinogen by the developed antibodies.

Conclusions:

  • The novel strategy enables highly efficient isolation of conformation-specific antibodies.
  • The developed antibodies are potent antagonists of activated Mac-1, inhibiting key neutrophil functions.
  • This approach is broadly applicable for discovering antibody antagonists against conformation-switching protein domains for therapeutic and diagnostic use.