Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview01:02

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material,  molecules absorb light depending on the energy required for electronic transitions. As a result...
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
UV–Vis Spectroscopy: Beer–Lambert Law01:09

UV–Vis Spectroscopy: Beer–Lambert Law

The Beer-Lambert law describes the relationship between absorbance and concentration, which combines the principles established by scientists Johann Heinrich Lambert and August Beer. Lambert's law states that when light passes through a medium, the loss in intensity is directly proportional to the original intensity and the path length of the light. Beer's law proposed that the transmittance of a solution remains constant if the product of concentration and path length is constant. The modern...
UV–Vis Spectrum01:30

UV–Vis Spectrum

When light passes through a substance, a portion of the light is absorbed while the remaining light is reflected or transmitted. If the molecule absorbs light between the wavelengths of 180–400 nm range, the UV spectrum is obtained, and if it absorbs light in the 400–780 nm wavelength range, the visible spectrum is obtained.     
The UV–Vis spectrum of a molecule is the plot of its absorbance versus wavelength. The plot is drawn by taking molar absorptivity (ε) or log ε on the y-axis (ordinate)...
UV–Vis Spectroscopy: Molecular Electronic Transitions01:16

UV–Vis Spectroscopy: Molecular Electronic Transitions

In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this process,...
UV–Vis Spectroscopy of Conjugated Systems01:32

UV–Vis Spectroscopy of Conjugated Systems

Organic compounds with conjugated double bonds show strong absorption features in the UV–visible region of the electromagnetic spectrum attributed to π → π* electronic excitations. Generally, a UV–vis absorption spectrum is recorded as a plot of absorbance vs wavelength. The wavelength of maximum absorbance, which manifests as a peak in the absorption spectrum, is denoted as λmax.
One of the factors influencing λmax is the extent of conjugation in the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Investigating the fluid and electrolyte prescribing knowledge of Foundation Year doctors.

Annals of the Royal College of Surgeons of England·2025
Same author

Hybrid closed loop technology in emergency surgery in a person with type 1 diabetes.

Anaesthesia reports·2025
Same author

Opioid stewardship.

BJA education·2023
Same author

Risks and benefits of oral modified-release compared with oral immediate-release opioid use after surgery: a systematic review and meta-analysis.

Anaesthesia·2023
Same author

Impact of modified-release opioid use on clinical outcomes following total hip and knee arthroplasty: a propensity score-matched cohort study.

Anaesthesia·2023
Same author

The prescribed opioid crisis as an impetus to improve postoperative pain management.

Anaesthesia·2023

Related Experiment Video

Updated: Jun 14, 2026

UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media
05:16

UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media

Published on: October 25, 2021

Ultraviolet analysis of graded-index lightguide preforms.

N Levy

    Applied Optics
    |March 24, 2010
    PubMed
    Summary

    Researchers identified a new UV absorption band at 330 nm in optical fiber preforms, linked to amorphous germanium dioxide (GeO2). This finding explains why phosphosilicate fibers are more susceptible to UV damage than borosilicate ones.

    Area of Science:

    • Materials Science
    • Optical Physics
    • Spectroscopy

    Background:

    • Graded-index fiber-optic preforms are crucial for telecommunications.
    • Understanding UV absorption properties is vital for fiber durability.
    • Previous studies lacked resolution for specific absorption bands.

    Purpose of the Study:

    • To investigate the UV absorption spectra of optical fiber preforms.
    • To identify and assign previously unresolved absorption bands.
    • To correlate absorption characteristics with UV damage susceptibility.

    Main Methods:

    • Utilized a UV absorption spectrophotometry technique with annular masks.
    • Excited selected regions of preforms with a collimated UV beam.
    • Measured absorption spectra across varying mask diameters for germania borosilicate (GeBSi) and phosphosilicate (GePSi) preforms.

    More Related Videos

    Molecular Beam Mass Spectrometry With Tunable Vacuum Ultraviolet (VUV) Synchrotron Radiation
    09:53

    Molecular Beam Mass Spectrometry With Tunable Vacuum Ultraviolet (VUV) Synchrotron Radiation

    Published on: October 30, 2012

    Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems
    09:57

    Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

    Published on: February 10, 2020

    Related Experiment Videos

    Last Updated: Jun 14, 2026

    UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media
    05:16

    UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media

    Published on: October 25, 2021

    Molecular Beam Mass Spectrometry With Tunable Vacuum Ultraviolet (VUV) Synchrotron Radiation
    09:53

    Molecular Beam Mass Spectrometry With Tunable Vacuum Ultraviolet (VUV) Synchrotron Radiation

    Published on: October 30, 2012

    Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems
    09:57

    Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

    Published on: February 10, 2020

    Main Results:

    • Discovered and assigned a new absorption band at 330 nm to amorphous germanium dioxide (GeO2).
    • Correlated peak absorption coefficients with X-ray fluorescence measurements of GeO2 concentrations.
    • Observed strong UV absorption in GePSi preforms below 320 nm, contrasting with weak absorption in GeBSi preforms.

    Conclusions:

    • The 330 nm band is definitively linked to amorphous GeO2 in optical fiber preforms.
    • Differences in UV absorption between GePSi and GeBSi preforms are significant below 320 nm.
    • The higher UV damage sensitivity of coated GePSi fibers is attributed to their absorption characteristics and UV-cured coating properties.