Related Experiment Video

Updated: Jun 14, 2026

A Stable Phantom Material for Optical and Acoustic Imaging
04:54

A Stable Phantom Material for Optical and Acoustic Imaging

Published on: June 16, 2023

Comparison of reflectance with backscatter and absorption parameters for turbid waters

C H Whitlock, L R Poole, J W Usry

    Applied Optics
    |March 24, 2010
    PubMed

    More Related Videos

    Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared07:38

    Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared

    Published on: January 10, 2025

    Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging07:06

    Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging

    Published on: May 7, 2017

    Related Concept Videos

    Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

    Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

    Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
    The ATR process begins by directing a beam...
    Total Internal Reflection Fluorescence Microscopy01:05

    Total Internal Reflection Fluorescence Microscopy

    Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
    UV–Vis Spectroscopy: Beer–Lambert Law01:09

    UV–Vis Spectroscopy: Beer–Lambert Law

    The Beer-Lambert law describes the relationship between absorbance and concentration, which combines the principles established by scientists Johann Heinrich Lambert and August Beer. Lambert's law states that when light passes through a medium, the loss in intensity is directly proportional to the original intensity and the path length of the light. Beer's law proposed that the transmittance of a solution remains constant if the product of concentration and path length is constant. The modern...
    Spectrophotometry: Introduction01:16

    Spectrophotometry: Introduction

    Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
    The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
    UV–Vis Spectrometers01:14

    UV–Vis Spectrometers

    The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...