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Updated: Jun 14, 2026

Fluorescence detection methods for microfluidic droplet platforms
Published on: December 10, 2011
Abstract:
A six-flux theory is derived for the diffuse reflectance and fluorescence of mixed powder samples of various shades of gray. One component of the powder is assumed to be fluorescent, with nonoverlapping excitation and emission bands. For symmetrical normal illumination and isotropic scattering, the six-flux reflectance reduces exactly to the Kubelka-Munk (two-flux) expression. Measurements of the fluorescence of a series of silica-silicon carbide-calcium tungstate mixed powder samples, which were found to vary over a range of 60:1, correlate well with the six-flux theory over the full range, whereas a two-flux theory is satisfactory only at high sample reflectances.
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